Plant cell, Tissue and Organ Culture: Fundamental Methods with 86 Figures

By: Gamborg, O.LContributor(s): G.C.Phillips (Editor)Material type: TextTextPublication details: New Delhi: Narosa Pub, 1996Description: xxiv, 348 pagesISBN: 8173191018 Subject(s): Plant cell culture -- Laboratory manuals | Plant tissue culture -- Laboratory manuals | Plant micropropagation -- Laboratory manuals | View all subjectsDDC classification: 581.0724
Contents:
Facilities and Technology Requirements for Laboratory Operations -- Laboratory Facilities, Operation, and Management -- Media Preparation and Handling -- Sterile Techniques -- Basic Plant Regeneration and Plant Propagation Procedures -- Micropropagation by Proliferation of Axillary Buds -- Adventitious Shoot Proliferation -- Plant Regeneration by Organogenesis from Callus and Cell Suspension Cultures -- Plant Regeneration from Callus and Cell Suspension Cultures by Somatic Embryogenesis -- Direct Somatic Embryogenesis -- Conifer Somatic Embryogenesis, Embryo Development, Maturation Drying, and Plant Formation -- Applications of Organ Culture Systems -- Meristem Culture for Virus Elimination -- Embryo Culture and Embryo Rescue for Wide Cross Hybrids -- Anther Culture for Doubled Haploid Production -- Microspore Culture for Haploid Plant Production -- Protoplasts and Transformation Procedures -- Protoplast Isolation and Culture -- Agrobacterium-Mediated Transformation -- Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment -- Microtechniques and Analytical Procedures -- Botanical Microtechnique for Plant Cultures -- Transmission and Scanning Electron Microscopy for Plant Protoplasts, Cultured Cells and Tissues -- Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts -- Staining Procedures for Chromosome Analysis -- Enzyme-Linked Immunosorbent Assay (ELISA) Methods to Certify Pathogen (Virus)-Free Plants -- Applications of Polymerase Chain Reaction (PCR) to Plant Genome Analysis.
Summary: A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. Also included are chapters on laboratory facilities, operations and procedures.
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Item type Current library Collection Call number Status Date due Barcode Item holds
Reference Books Reference Books Main Library
Reference 		Permanent Reference 581.0724 PLA (Browse shelf(Opens below)) Available 005270
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Included Index

Facilities and Technology Requirements for Laboratory Operations --
Laboratory Facilities, Operation, and Management --
Media Preparation and Handling --
Sterile Techniques --
Basic Plant Regeneration and Plant Propagation Procedures --
Micropropagation by Proliferation of Axillary Buds --
Adventitious Shoot Proliferation --
Plant Regeneration by Organogenesis from Callus and Cell Suspension Cultures --
Plant Regeneration from Callus and Cell Suspension Cultures by Somatic Embryogenesis --
Direct Somatic Embryogenesis --
Conifer Somatic Embryogenesis, Embryo Development, Maturation Drying, and Plant Formation --
Applications of Organ Culture Systems --
Meristem Culture for Virus Elimination --
Embryo Culture and Embryo Rescue for Wide Cross Hybrids --
Anther Culture for Doubled Haploid Production --
Microspore Culture for Haploid Plant Production --
Protoplasts and Transformation Procedures --
Protoplast Isolation and Culture --
Agrobacterium-Mediated Transformation --
Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment --
Microtechniques and Analytical Procedures --
Botanical Microtechnique for Plant Cultures --
Transmission and Scanning Electron Microscopy for Plant Protoplasts, Cultured Cells and Tissues --
Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts --
Staining Procedures for Chromosome Analysis --
Enzyme-Linked Immunosorbent Assay (ELISA) Methods to Certify Pathogen (Virus)-Free Plants --
Applications of Polymerase Chain Reaction (PCR) to Plant Genome Analysis.

A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. Also included are chapters on laboratory facilities, operations and procedures.

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