An Introduction to Genetic Engineering (Record no. 35927)
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000 -LEADER | |
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fixed length control field | 02972nam a2200157 a 4500 |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
ISBN | 0521586917 |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
Classification number | 574.10724 |
Item number | NIC |
100 ## - MAIN ENTRY--AUTHOR NAME | |
Personal name | Nicholl, Desmond S.T; |
245 ## - TITLE STATEMENT | |
Title | An Introduction to Genetic Engineering |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Place of publication | Cambridge: |
Name of publisher | Cambridge University Press, |
Year of publication | 1996. |
300 ## - PHYSICAL DESCRIPTION | |
Number of Pages | x, 168 pages. |
Other physical details | illustration. |
500 ## - GENERAL NOTE | |
General note | included Glossary and Index |
505 ## - FORMATTED CONTENTS NOTE | |
Formatted contents note | The basis of genetic engineering --<br/>Introducing molecular biology --<br/>The flow of genetic information --<br/>The structure of DNA and RNA --<br/>Gene organisation --<br/>Gene structure in prokaryotes --<br/>Gene structure in eukaryotes --<br/>Gene expression --<br/>Genes and genomes --<br/>Genome size and complexity --<br/>Genome organisation --<br/>Working with nucleic acids --<br/>Isolation of DNA and RNA --<br/>Handling and quantification of nucleic acids --<br/>Radiolabelling of nucleic acids --<br/>End labelling --<br/>Nick translation --<br/>Labelling by primer extension --<br/>Nucleic acid hybridisation --<br/>Gel electrophoresis --<br/>DNA sequencing --<br/>Maxam--Gilbert (chemical) sequencing --<br/>Sanger--Coulson (dideoxy or enzymatic) sequencing --<br/>Electrophoresis and reading of sequences --<br/>The tools of the trade --<br/>Restriction enzymes--cutting DNA --<br/>Type II restriction endonucleases --<br/>Use of restriction endonucleases --<br/>Restriction mapping --<br/>DNA modifying enzymes --<br/>Nucleases --<br/>Polymerases --<br/>Enzymes that modify the ends of DNA molecules --<br/>DNA ligase--joining DNA molecules --<br/>The methodology of gene manipulation --<br/>Host cells and vectors --<br/>Host cell types --<br/>Prokaryotic hosts --<br/>Eukaryotic hosts --<br/>Plasmid vectors for use in E. coli --<br/>What are plasmids? --<br/>Basic cloning plasmids --<br/>Slightly more exotic plasmid vectors --<br/>Bacteriophage vectors for use in E. coli --<br/>What are bacteriophages? --<br/>Vectors based on bacteriophage [lambda] --<br/>Vectors based on bacteriophage M13 --<br/>Other vectors --<br/>Hybrid plasmid/phage rectors --<br/>Vectors for use in eukaryotic cells --<br/>Artificial chromosomes. |
520 ## - SUMMARY, ETC. | |
Summary, etc | Des Nicholl presents here a new, fully revised, and expanded edition of his popular undergraduate-level textbook. Many of the features of the original edition have been retained; the book still offers a concise technical introduction to the subject of genetic engineering. However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic engineering. A whole chapter is now devoted to the polymerase chain reaction. Applications covered in the book include genomics, protein engineering, gene therapy, cloning, and transgenic animals and plants. A final chapter discusses the ethical questions surrounding genetic engineering in general. An Introduction to Genetic Engineering is essential reading for undergraduate students of biotechnology, genetics, molecular biology and biochemistry. |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Lending Books |
Collection code | Home library | Current library | Shelving location | Date acquired | Source of acquisition | Cost, normal purchase price | Full call number | Accession Number | Koha item type |
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Main Library | Main Library | Stacks | 25/05/1998 | Purchased | 225.00 | 574.10724 NIC | 004574 | Lending Books |