An Introduction to Genetic Engineering (Record no. 35927)

MARC details
000 -LEADER
fixed length control field 02972nam a2200157 a 4500
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
ISBN 0521586917
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 574.10724
Item number NIC
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Nicholl, Desmond S.T;
245 ## - TITLE STATEMENT
Title An Introduction to Genetic Engineering
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication Cambridge:
Name of publisher Cambridge University Press,
Year of publication 1996.
300 ## - PHYSICAL DESCRIPTION
Number of Pages x, 168 pages.
Other physical details illustration.
500 ## - GENERAL NOTE
General note included Glossary and Index
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note The basis of genetic engineering --<br/>Introducing molecular biology --<br/>The flow of genetic information --<br/>The structure of DNA and RNA --<br/>Gene organisation --<br/>Gene structure in prokaryotes --<br/>Gene structure in eukaryotes --<br/>Gene expression --<br/>Genes and genomes --<br/>Genome size and complexity --<br/>Genome organisation --<br/>Working with nucleic acids --<br/>Isolation of DNA and RNA --<br/>Handling and quantification of nucleic acids --<br/>Radiolabelling of nucleic acids --<br/>End labelling --<br/>Nick translation --<br/>Labelling by primer extension --<br/>Nucleic acid hybridisation --<br/>Gel electrophoresis --<br/>DNA sequencing --<br/>Maxam--Gilbert (chemical) sequencing --<br/>Sanger--Coulson (dideoxy or enzymatic) sequencing --<br/>Electrophoresis and reading of sequences --<br/>The tools of the trade --<br/>Restriction enzymes--cutting DNA --<br/>Type II restriction endonucleases --<br/>Use of restriction endonucleases --<br/>Restriction mapping --<br/>DNA modifying enzymes --<br/>Nucleases --<br/>Polymerases --<br/>Enzymes that modify the ends of DNA molecules --<br/>DNA ligase--joining DNA molecules --<br/>The methodology of gene manipulation --<br/>Host cells and vectors --<br/>Host cell types --<br/>Prokaryotic hosts --<br/>Eukaryotic hosts --<br/>Plasmid vectors for use in E. coli --<br/>What are plasmids? --<br/>Basic cloning plasmids --<br/>Slightly more exotic plasmid vectors --<br/>Bacteriophage vectors for use in E. coli --<br/>What are bacteriophages? --<br/>Vectors based on bacteriophage [lambda] --<br/>Vectors based on bacteriophage M13 --<br/>Other vectors --<br/>Hybrid plasmid/phage rectors --<br/>Vectors for use in eukaryotic cells --<br/>Artificial chromosomes.
520 ## - SUMMARY, ETC.
Summary, etc Des Nicholl presents here a new, fully revised, and expanded edition of his popular undergraduate-level textbook. Many of the features of the original edition have been retained; the book still offers a concise technical introduction to the subject of genetic engineering. However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic engineering. A whole chapter is now devoted to the polymerase chain reaction. Applications covered in the book include genomics, protein engineering, gene therapy, cloning, and transgenic animals and plants. A final chapter discusses the ethical questions surrounding genetic engineering in general. An Introduction to Genetic Engineering is essential reading for undergraduate students of biotechnology, genetics, molecular biology and biochemistry.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Lending Books
Holdings
Collection code Home library Current library Shelving location Date acquired Source of acquisition Cost, normal purchase price Full call number Accession Number Koha item type
  Main Library Main Library Stacks 25/05/1998 Purchased 225.00 574.10724 NIC 004574 Lending Books

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