An Introduction to Genetic Engineering

By: Nicholl, Desmond S.TMaterial type: TextTextPublication details: Cambridge: Cambridge University Press, 1996Description: x, 168 pages. illustrationISBN: 0521586917DDC classification: 574.10724
Contents:
The basis of genetic engineering -- Introducing molecular biology -- The flow of genetic information -- The structure of DNA and RNA -- Gene organisation -- Gene structure in prokaryotes -- Gene structure in eukaryotes -- Gene expression -- Genes and genomes -- Genome size and complexity -- Genome organisation -- Working with nucleic acids -- Isolation of DNA and RNA -- Handling and quantification of nucleic acids -- Radiolabelling of nucleic acids -- End labelling -- Nick translation -- Labelling by primer extension -- Nucleic acid hybridisation -- Gel electrophoresis -- DNA sequencing -- Maxam--Gilbert (chemical) sequencing -- Sanger--Coulson (dideoxy or enzymatic) sequencing -- Electrophoresis and reading of sequences -- The tools of the trade -- Restriction enzymes--cutting DNA -- Type II restriction endonucleases -- Use of restriction endonucleases -- Restriction mapping -- DNA modifying enzymes -- Nucleases -- Polymerases -- Enzymes that modify the ends of DNA molecules -- DNA ligase--joining DNA molecules -- The methodology of gene manipulation -- Host cells and vectors -- Host cell types -- Prokaryotic hosts -- Eukaryotic hosts -- Plasmid vectors for use in E. coli -- What are plasmids? -- Basic cloning plasmids -- Slightly more exotic plasmid vectors -- Bacteriophage vectors for use in E. coli -- What are bacteriophages? -- Vectors based on bacteriophage [lambda] -- Vectors based on bacteriophage M13 -- Other vectors -- Hybrid plasmid/phage rectors -- Vectors for use in eukaryotic cells -- Artificial chromosomes.
Summary: Des Nicholl presents here a new, fully revised, and expanded edition of his popular undergraduate-level textbook. Many of the features of the original edition have been retained; the book still offers a concise technical introduction to the subject of genetic engineering. However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic engineering. A whole chapter is now devoted to the polymerase chain reaction. Applications covered in the book include genomics, protein engineering, gene therapy, cloning, and transgenic animals and plants. A final chapter discusses the ethical questions surrounding genetic engineering in general. An Introduction to Genetic Engineering is essential reading for undergraduate students of biotechnology, genetics, molecular biology and biochemistry.
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Item type Current library Collection Call number Status Date due Barcode Item holds
Lending Books Lending Books Main Library
Stacks 		REF 574.10724 NIC (Browse shelf(Opens below)) Available 004574
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included Glossary and Index

The basis of genetic engineering --
Introducing molecular biology --
The flow of genetic information --
The structure of DNA and RNA --
Gene organisation --
Gene structure in prokaryotes --
Gene structure in eukaryotes --
Gene expression --
Genes and genomes --
Genome size and complexity --
Genome organisation --
Working with nucleic acids --
Isolation of DNA and RNA --
Handling and quantification of nucleic acids --
Radiolabelling of nucleic acids --
End labelling --
Nick translation --
Labelling by primer extension --
Nucleic acid hybridisation --
Gel electrophoresis --
DNA sequencing --
Maxam--Gilbert (chemical) sequencing --
Sanger--Coulson (dideoxy or enzymatic) sequencing --
Electrophoresis and reading of sequences --
The tools of the trade --
Restriction enzymes--cutting DNA --
Type II restriction endonucleases --
Use of restriction endonucleases --
Restriction mapping --
DNA modifying enzymes --
Nucleases --
Polymerases --
Enzymes that modify the ends of DNA molecules --
DNA ligase--joining DNA molecules --
The methodology of gene manipulation --
Host cells and vectors --
Host cell types --
Prokaryotic hosts --
Eukaryotic hosts --
Plasmid vectors for use in E. coli --
What are plasmids? --
Basic cloning plasmids --
Slightly more exotic plasmid vectors --
Bacteriophage vectors for use in E. coli --
What are bacteriophages? --
Vectors based on bacteriophage [lambda] --
Vectors based on bacteriophage M13 --
Other vectors --
Hybrid plasmid/phage rectors --
Vectors for use in eukaryotic cells --
Artificial chromosomes.

Des Nicholl presents here a new, fully revised, and expanded edition of his popular undergraduate-level textbook. Many of the features of the original edition have been retained; the book still offers a concise technical introduction to the subject of genetic engineering. However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic engineering. A whole chapter is now devoted to the polymerase chain reaction. Applications covered in the book include genomics, protein engineering, gene therapy, cloning, and transgenic animals and plants. A final chapter discusses the ethical questions surrounding genetic engineering in general. An Introduction to Genetic Engineering is essential reading for undergraduate students of biotechnology, genetics, molecular biology and biochemistry.

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